We just pushed an update to fix the issue of mods disabling. Please make sure the mods your city needs are enabled, then restart the game and check they're still enabled before loading your city.
Originally posted by Andy Er: This patch destroyed the game. Modded CS does not start. Game runs without problems if param --noWorkshop is used
If you use mods please check your mods against the list of broken mods, both the first tab and especially the tab named Patch 1.16.1.
https://pdxint.at/BrokenModCS
If that doesn't help or you don't use mods, please make a thread on our support forum. We need to see your output_log.txt (called player.log on Mac and Linux) to be able to help, which cannot be attached here on Steam.
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